Formulations and methods for control of weedy species
AssigneeTerramera, Inc.
InventorsChris Tuttle, Layne Woodfin
Life of this patent
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Abstract
A formulation is provided for application to a host plant to reduce, inhibit or impair one or more of growth and development of the host plant. A method of inhibiting growth plant growth and development is also provided as a means of controlling weedy species. The method comprises: selecting a suitable gene for growth suppression in a target plant; identifying an at least one target site accessible to base pairing in the suitable gene; identifying an at least one divergent site in the at least one target site; designing a construct complementary to the at least one divergent site; adding an at least one RNAi inducer to the construct; and delivering the construct to the target plant.
Claims24
A method of designing a species-specific gene construct for RNAi suppression of growth of a target plant species, the method comprising the steps of:
- selecting at least two target genes for growth suppression, wherein one of said at least two target genes is Beclin1, Accelerated cell death 2 (Acd2), Accelerated cell death 11 (Acd11), Catalase 1 (Cat1), Lesion stimulating disease 1 (Lsd1), Bax inhibitor 1 (BI-1), Lethal leaf spot 1-like (Lls1), Metacaspase 2 (MC2), said genes being from Nicotiana sylvestris or Nicotiana tobacum or having at least 95% nucleotide sequence identity to said genes from either one of Nicotiana sylvestris or Nicotiana tobacum across each one of the at least two target genes;
- identifying at least one target site accessible to base pairing in each one of the at least two target genes;
- identifying at least one divergent site in each one of the at least one target sites;
- designing a nucleotide sequence targeting construct complementary to each one of the at least one divergent sites, the nucleotide sequence targeting construct being at least 21 nucleotides in length and being complementary to the at least one divergent site across at least 21 contiguous nucleotides;
- and
- incorporating at least one RNAi inducer to the nucleotide sequence construct, thereby designing a RNAi payload for RNAi suppression of growth of the target plant species.
A method of inhibiting or impairing growth and development of a target plant, the method comprising:
- selecting at least two target genes for growth suppression, wherein one of said at least two target genes is Beclin1, Accelerated cell death 2 (Acd2), Accelerated cell death 11 (Acd11), Catalase 1 (Cat1), Lesion stimulating disease 1 (Lsd1), Bax inhibitor 1 (BI-1), Lethal leaf spot 1-like (Lls1), Metacaspase 2 (MC2), said genes being from Nicotiana sylvestris or Nicotiana tobacum or having at least 95% nucleotide sequence identity to said genes from either one of Nicotiana sylvestris or Nicotiana tobacum across each one of the at least two target genes;
- identifying at least one target site accessible to base pairing in each one of the at least two target genes;
- identifying at least one divergent site in each one of the at least one target sites;
- designing a nucleotide sequence targeting construct complementary to each one of the at least one divergent sites, the nucleotide sequence targeting construct being at least 21 nucleotides in length and being complementary to the at least one divergent site across at least 21 contiguous nucleotides;
- adding at least one RNAi inducer to the nucleotide sequence construct thereby producing a RNAi payload;
- and
- delivering the RNAi payload to the target plant.
Dependencies are inferred from the claim text.