Synthetic nucleic acids having non-natural structures
AssigneePacific Biosciences of California, Inc.
InventorsArnold Oliphant, James Yu
Life of this patent
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Abstract
Nucleic acid compositions having nucleotide sequences that do not occur in nature are provided. Also provided are populations of different nucleic acids having universal adapters or universal primer binding sites. Methods of capturing, copying or amplifying nucleic acids of interest using universal adapters, universal primer binding sites and/or universal primers are also provided.
Claims23
A composition comprising an isolated nucleic acid molecule comprising the sequence or the complement of SEQ ID NO:1.
A population of different nucleic acid molecules, comprising a plurality of nucleic acid molecules,
- wherein each of the nucleic acid molecules comprises a universal sequence region and a variable sequence region,
- wherein the sequence of the variable sequence region differs between different nucleic acid molecules in the population,
- wherein the sequence of the universal sequence region is the same for the nucleic acid molecules in the population, and
- wherein the universal sequence region comprises the sequence or the complement of SEQ ID NO:1.
A method for preparing a nucleic acid library, comprising
- attaching universal adapters to nucleic acid fragments from a genome, thereby producing a library of nucleic acid molecules,
- wherein each of the nucleic acid molecules in the library comprises:
- (i) a variable sequence region that differs between the nucleic acid molecules in the library, and
- (ii) a universal sequence region that is the same for the nucleic acid molecules in the library, wherein the universal sequence region comprises the sequence or the complement of SEQ ID NO:1.
Dependencies are inferred from the claim text.