Method for extracting DNA
AssigneeFüLl Process S.A.
InventorsJoel Johannes Frank, Thomas Brinz
Life of this patent
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Abstract
The invention relates to a method for extracting DNA, comprising the steps of: providing (400) a lysate (11) in a sample vessel (1), feeding (500) an DNA absorbing substance (15) to the sample vessel (1), closing (700) the sample vessel (1) by means of a wash filter element (3), feeding (600) a wash fluid (12) to the sample vessel (1) through the wash filter element (3), and discharging (700) the wash fluid (12) from the sample vessel (1) through the wash filter element (3). The DNA absorbing substance can be retained from the wash filter element (3).
Claims20
A method for extracting DNA, comprising the steps:
- lysing cells in a first sample vessel (2) to form a lysate,
- filtering the lysate through a lysate filter element (4) into a second sample vessel (1) to form a filtered lysate (11) comprising DNA released from the cells,
- adding a DNA-adsorbing substance (15) to the second sample vessel (1) comprising the DNA released from the cells, wherein the DNA-adsorbing substance binds to and forms a complex with at least some of the DNA released from the cells,
- closing the second sample vessel (1) with a wash filter element (3) formed of the same material as the lysate filter element (4),
- delivering a wash fluid (12) into the second sample vessel (1) through the wash filter element (3), and
- discharging the wash fluid (12) from the second sample vessel (1) through the wash filter element (3), wherein the complex is retained within the second sample vessel (1) by the wash filter element (3), and wherein any DNA that is not bound to the DNA-adsorbing substance is not retained by the wash filter element (3),
- introducing an additional substance into the second sample vessel (1) in order to separate the DNA-adsorbing substance (15) from the DNA, producing free DNA, and
- separating the DNA-adsorbing substance from the free DNA in the second sample vessel (1), leaving the free DNA in the second sample vessel (1).
Dependencies are inferred from the claim text.