US 11,377,635 B2 · Highly efficient gas permeable devices and methods for culturing cells

Abstract

This invention relates to methods and devices that improve cell culture efficiency. They include the use of gas permeable culture compartments that reduce the use of space while maintaining uniform culture conditions, and are more suitable for automated liquid handling. They include the integration of gas permeable materials into the traditional multiple shelf format to resolve the problem of non-uniform culture conditions. They include culture devices that use surfaces comprised of gas permeable, plasma charged silicone and can integrate traditional attachment surfaces, such as those comprised of traditional tissue culture treated polystyrene. They include culture devices that integrate gas permeable, liquid permeable membranes. A variety of benefits accrue, including more optimal culture conditions during scale up and more efficient use of inventory space, incubator space, and disposal space. Furthermore, labor and contamination risk are reduced.

Claims21

Dependent claims

A static cell growth apparatus, comprising:

a housing that is liquid impermeable and capable of holding medium and including a plurality of gas permeable shelves for cells to reside upon, wherein:
each gas permeable shelf has an opposing surface, the space between each gas permeable shelf and its opposing surface being a cell culture compartment, wherein each cell culture compartment has the same volume of space and the space is capable of being filled with medium;
the outside surface of each gas permeable shelf is in contact with projections that are spaced apart and are configured to create a space for incubator gas to contact the outside surface of each gas permeable shelf and to maintain each gas permeable shelf in a horizontal position during cell growth;
and
the culture compartments reside one above the other when the shelves are in a horizontal position;
and
a manifold connecting each culture compartment.

4 more elements

Dependencies are inferred from the claim text.

Highly efficient gas permeable devices and methods for culturing cells

Life of this patent

Highly efficient gas permeable devices and methods for culturing cells

Life of this patent

Abstract

Claims21