Medium supplement for high-yield industrial culture of fastidious anaerobes and medium composition containing the same
AssigneeEnterobiome Inc.
InventorsJae-Gu Seo, Do-Kyung Lee
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Abstract
A medium supplement for high-yield culture of anaerobes is disclosed. The medium supplement includes N-acetylhexosamine, L-aspartic acid, L-cysteine and cobalamin. A culture medium including the supplement and a culture method using the culture medium are also disclosed. It is possible to provide an innovative method which is capable of achieving high-concentration culture of anaerobes that are difficult to culture in high yield. The method is cost-effective, and in particular, is capable of culturing large amounts of fastidious aerobes suitable for use in food and pharmaceutical applications.
Claims11
A medium composition for culture of anaerobes, the medium composition comprising:
- plant peptone;
- yeast extract;
- potassium phosphate dibasic;
- as a carbon source, fructose and lactose;
- and as supplements, N-acetylhexosamine, an amino acid mixture of L-aspartic acid and L-cysteine, and cobalamin,
- wherein the anaerobes are selected from the group consisting of Faecalibacterium prausnitzii, Anaerostipes caccae, Akkermansia muciniphila, and Bifidobacterium longum.
Dependencies are inferred from the claim text.