Cell culture kit

A cell culture kit comprising:

• an external container having an open top and a closed base to provide an accommodation space therein;
• at least one cell culture film detachably stacked inside the external container;
• and
• a cover portion coupled to the open top of the external container,
• wherein the at least one cell culture film includes:
• a first film disposed within the external container;
• and
• a second film disposed between the first film and the base of the external container, the second film detachably adhering to the first film,
• wherein the first film includes:
• a first top surface on which a cell is cultured;
• a first bottom surface facing the second film;
• and
• a plurality of first culture holes formed at a body of the first film, the plurality of first culture holes passing through the first top and bottom surfaces of the first film,
• wherein the second film includes:
• second top and bottom surfaces facing the first bottom surface of the first film and the base of the external container, respectively;
• and
• a plurality of second culture holes formed at a body of the second film, the plurality of second culture holes passing through the second top and bottom surfaces of the second film,
• wherein the second top surface of the second film contacts the first bottom surface of the first film and the plurality of first culture holes do not overlap with the plurality of second culture holes, such that the cell on the first top surface is transferred and cultured on the second top surface which is exposed via the plurality of the first culture holes,
• wherein the second film further includes a culture groove formed at a body of the second film and protruding downwardly toward the base of the external container, the culture groove having an open top and a closed bottom to form an inner space and accommodating a cell assembly in the inner space to be cultured,
• wherein the at least one cell culture film includes a tunnel formed from the first top surface of the first film to the inner space of the culture groove of the second film.