Co-cultivation of a myxobacterium and acinetobacter for enhanced production of emulsan
AssigneeLocus IP Company, LLC
InventorsSean Farmer, Ken Alibek, Yajie Chen
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Abstract
Methods are provided for enhanced production of one or more microbial biopolymers, the methods comprising co-cultivating Stigmatella aurantiaca and Acinetobacter venetianus. In certain embodiments, the one or more biopolymers are emulsan. In certain embodiments, other microbial growth by-products are produced, such as biosurfactants. Microbe-based products produced according to the subject methods are also provided, as well as their uses in, for example, oil and gas recovery, agriculture, and health care.
Claims8
A method for enhanced production of one or more microbial growth by-products selected from the group consisting of biopolymers and biosurfactants, the method comprising co-cultivating a first microorganism and a second microorganism in a fermentation reactor comprising a liquid nutrient medium and incubating the microorganisms in the reactor under conditions favorable for growth and production of the one or more microbial growth by-products, feeding clear liquefied paraffin into the reactor after 24 hours of incubation, extracting the one or more growth by-products from the reactor, and, optionally, purifying the one or more growth by-products,
- wherein the first microorganism is Stigmatella aurantiaca and the second microorganism is Acinetobacter venetianus RAG-1,
- wherein the biopolymer is emulsan and the biosurfactant is a glycolipid or a lipopeptide, and
- wherein a greater concentration of the one or more microbial growth by-products is achieved than would be achieved if the first and second microorganisms were cultivated individually.
Dependencies are inferred from the claim text.