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AssigneeCell Signaling Technology, Inc.
InventorsMichael Comb, Ailan Guo, John Edward Rush, II, Jun-Ming Cai, Jing Li, Jing Zhou
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There is provided a motif-specific, context-independent antibody that specifically binds a recurring, modified motif consisting of (i) at least one sumoylated lysine residue, and (ii) one or more degenerate amino acids bound by a peptide bond to said sumoylated lysine residue, said antibody specifically binding said motif in a plurality of non-homologous peptides or proteins within an organism in which it recurs. Also provided is a motif-specific, context-independent antibody that specifically binds a recurring, modified motif consisting of (i) a C-terminal aspartic acid residue, and (ii) one or more degenerate amino acids bound by a peptide bond to said C-terminal aspartic acid residue, said antibody specifically binding said motif in a plurality of non-homologous peptides or proteins within an organism in which it recurs.
A method of producing a purified motif-specific, context-independent antibody that specifically binds a motif comprising (i) a sequence of ψ-K-X-D, wherein ψ is a hydrophobic amino acid residue, K is a sumoylated lysine residue, X is any amino acid residue, and D is an aspartic acid residue, and (ii) one or more degenerate amino acids bound by a peptide bond to the sumoylated lysine residue, wherein the method comprises (a) constructing a degenerate peptide library comprising (i) the motif and (ii) a plurality of degenerate amino acids flanking said motif;
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