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US 11,377,470 B2Granted
Ribonucleic acid purification
AssigneeModernaTX, Inc.
InventorsWilliam Joseph Issa, John Grant Aunins, Stephane Bancel
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Abstract
Disclosed herein are methods for purifying RNA comprising poly A. Also disclosed herein are compositions such as surfaces and oligonucleotides for purifying RNA comprising polyA. Other embodiments are also disclosed. Commercially-available resins having polythymidine oligonucleotide ligands typically contain less than 30 thymidine (2′deoxy) residues and some commercial resin suppliers utilize a distribution of dT chain lengths, not of a discreet length.
Claims17
A method for purifying a ribonucleic acid (RNA) transcript comprising a polyA tail, the method comprising:
- a) obtaining a first sample comprising the RNA transcript, wherein the first sample comprises RNA transcript and at least 5% impurities, and wherein the percentage of RNA transcript and the percentage of impurities are the inverse of each other;
- b) contacting the first sample with a surface linked to twenty thymidines via a linker, wherein the surface linked to twenty thymidines comprises the structure:
- or a salt thereof, under conditions such that the RNA transcript binds to the surface;
- c) eluting the RNA transcript from the surface;
- and
- d) collecting the RNA transcript in a second sample,
- wherein the level of impurities is lower in the second sample than the level of impurities in the first sample.
4 more elements
Dependencies are inferred from the claim text.