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US 11,376,593 B2Granted
Systems and methods for sorting T cells by activation state
AssigneeWisconsin Alumni Research Foundation
InventorsMelissa Caroline Skala, Alexandra Jule Walsh
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Abstract
Systems and methods for sorting T cells are disclosed. Autofluorescence data is acquired from individual cells. An activation value is computed using one or more autofluorescence endpoints as an input. The one or more autofluorescence endpoints includes NAD(P)H shortest fluorescence lifetime amplitude component (α1).
Claims10
A method of sorting T cells, the method comprising:
- a) receiving a population of T cells having unknown activation status;
- b) acquiring an autofluorescence data set for each T cell of the population of T cells, each autofluorescence data set including autofluorescence lifetime information;
- and
- c) physically isolating a first portion of the population of T cells from a second portion of the population of T cells based on an activation value, wherein each T cell of the population of T cells is placed into the first portion when the activation value exceeds a predetermined threshold and into the second portion when the activation value is less than or equal to the predetermined threshold, wherein the activation value is computed using at least one metabolic endpoint of the autofluorescence data set for each T cell of the population of T cells as an input, wherein the at least one metabolic endpoint includes reduced nicotinamide adenine dinucleotide (NAD(P)H) shortest fluorescence lifetime amplitude component (α1).
1 more element
Dependencies are inferred from the claim text.