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US 11,376,525 B2Granted
Three-port chamber for processing particles
AssigneeTerumo BCT, Inc.
InventorsTimothy Michael Taga, Anne Baker Oram, Linda A. Taylor, Dennis J. Hlavinka
Life of this patent
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Abstract
Embodiments relate to systems, chambers, and methods for processing particles by washing, concentrating, and/or treating the particles. Some embodiments provide for the processing of cells that may be in a liquid medium (e.g., a liquid in which the cells were grown) and are processed by being washed and concentrated for later use in research or other therapies.
Claims10
A method of processing particles, the method comprising:
- subjecting a chamber to a centrifugal field by rotating the chamber;
- introducing a first volume of particles and liquid into a chamber volume through a first port of the chamber;
- after introducing the first volume of particles and liquid into the chamber volum, introducing a second liquid into the chamber volume through a second port of the chamber, wherein the second port is positioned in a higher force region of the centrifugal field than the first port;
- removing liquid through a third port of the chamber, wherein the third port is positioned in a lower force region of the centrifugal field than the first port;
- and
- removing a second volume of particles and liquid through the second port, wherein the second volume of particles and liquid is smaller than the first volume of particles and liquid.
3 more elements
A method of processing particles, the method comprising:
- subjecting a chamber to a centrifugal field by rotating the chamber;
- introducing a first volume of particles and liquid into a chamber volume through a first port of the chamber;
- introducing a second liquid into the chamber volume through a second port of the chamber, wherein the second port is positioned in a higher force region of the centrifugal field than the first port;
- removing liquid through a third port of the chamber, wherein the third port is positioned in a lower force region of the centrifugal field than the first port;
- removing a second volume of particles and liquid through the second port, wherein the second volume of particles and liquid is smaller than the first volume of particles and liquid;
- and
- after the introducing the first volume of particles and liquid and before removing the second volume of particles and liquid, changing the viscosity of liquid in the chamber volume,
- wherein changing the viscosity comprises introducing the second liquid into the chamber volume through the first port, and
- wherein the second liquid is introduced through the first port at a first flow rate, which is greater than a flow rate of the second liquid introduced through the second port.
6 more elements
Dependencies are inferred from the claim text.