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US 11,376,338 B2Granted
Use of thermostable RNA polymerases to produce RNAs having reduced immunogenicity
AssigneeNew England Biolabs, Inc.
InventorsBijoyita Roy, G. B. Robb
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Abstract
Provided herein, among other things, is a method for producing an RNA product that has reduced immunogenicity. In some embodiments, the method involves transcribing a template DNA with a thermostable RNA polymerase at a temperature of greater than 44° C.
Claims11
A method for producing an RNA product with reduced immunogenicity, comprising:
- (a) combining a DNA and a thermostable RNA polymerase at a temperature of greater than 44° C., the RNA polymerase variant having an amino acid sequence that has at least 90% sequence identity with SEQ ID NO:1 and having an amino acid substitution at a position corresponding to position 567 and optionally at a position corresponding to position 388;
- and
- (b) producing the RNA product comprising a single strand RNA having reduced immunogenicity compared with an RNA product produced by an RNA polymerase at 37° C.
Dependencies are inferred from the claim text.