Sting activating nanovaccine for immunotherapy

A composition comprising:

• (A) a protein or peptide antigen;
• (B) a pH sensitive diblock copolymer nanoparticle, wherein the diblock copolymer is of the formula:
• wherein:
• R1 is hydrogen, alkyl(C<12), cycloalkyl(C<12), substituted alkyl(C<12), or substituted cycloalkyl(C<12);
• n is an integer from 1 to 500;
• R2 and R2′ are each independently selected from the group consisting of hydrogen, alkyl(C<12), cycloalkyl(C<12), substituted alkyl(C<12), and substituted cycloalkyl(C<12);
• R3 is a group of the formula:
• wherein:
• nx is 1-10;
• X1, X2, and X3 are each independently selected from hydrogen, alkyl(C<12), cycloalkyl(C<12), substituted alkyl(C<12), and substituted cycloalkyl(C<12);
• X4 and X5 are taken together and are —CH2CH2CH2CH2CH2—, —CH2CH2CH2CH2CH2CH2—, —CH2CH2CH(CH3)CH2CH2—, or —CH2CH(CH3)CH2CH(CH3)CH2—;
• x is an integer from 1 to 150;
• R4 is a group of the formula:
• wherein:
• ny is 1-10;
• X1′, X2′, and X3′ are each independently selected from hydrogen, alkyl(C<12), cycloalkyl(C<12), substituted alkyl(C<12), and substituted cycloalkyl(C<12);
• X4′ is a dye or a fluorescence quencher;
• X5′ is selected from hydrogen, alkyl(C<12), cycloalkyl(C<12), substituted alkyl(C<12), substituted cycloalkyl(C<12), substituted acyl(C<12), a dye, and a fluorescence quencher;
• y is an integer from 0 to 150;
• and
• R5 is hydrogen, halo, hydroxy, alkyl(C<12), or substituted alkyl(C<12);
• R3 and R4 can occur in any order within the diblock copolymer;
• and
• wherein the antigen is encapsulated by the diblock copolymer.