Microbial fermentation composition subjected to enzymolysis, microbial fermentation and microbial transformation and use thereof

A microbial fermentation composition, is prepared through extracting and hydrolyzing medicinal and edible fungi Agaricus blazei, Lentinus edodes, Flammulina velutipes and ginseng, adding trace elements selenium, zinc and molybdenum, and fermenting and transforming by probiotics:

• a weight g/volume mL percentage of the medicinal and edible fungi is:
• 1-3% of Agaricus blazei, 2-4% of Lentinus edodes, 1-4% of Flammulina velutipes;
• a weight g/volume mL percentage of the ginseng is 1-3%;
• an amount of the trace elements is:
• 30-200 μg/100 mL of sodium selenite, 20-85 mg/100 mL of zinc sulfate heptahydrate, and 50-150 μg/100 mL of sodium molybdate dihydrate;
• wherein the probiotics are Bifidobacterium and Lactobacillus;
• wherein a preparation method of the microbial fermentation composition is as follows:
• A. extraction of fermentation substrate:
• crushing dried ginseng, Agaricus blazei, Lentinus edodes, Flammulina velutipes, sieving through a 65-80 mesh sieve, adding drinking water that is 30 times the material weight and soaking for 1 hour, heating and boiling for 1.5 hours, and when reducing temperature to 90° C., adjusting a pH value between 5.5-7.0, adding high temperature amylase, adding an amount of 20 enzyme activity units×material weight/enzyme activity, maintaining the temperature between 80-90° C., and stirring evenly, ending amylase enzymolysis when material solution and iodine solution no longer turn blue, reducing the temperature of the material solution to 50° C., adjusting a pH value of the material solution to 5.0-6.0, adding pectin lyase, adding an amount of 0.1% volume of the material solution by volume/volume ratio, maintaining the temperature at 45-50° C., enzymolyzing for 2 hours, ending the enzymolyzing and boiling for 10 minutes, inactivating enzyme, filtering, and taking supernatant, concentrating under reduced pressure until concentration of Agaricus blazei, Lentinus edodes, Flammulina velutipes and ginseng reaches the concentration to obtain a fermentation substrate extract;
• B. ingredients and sterilization:
• adding sodium selenite, zinc sulfate, sodium molybdate to the fermentation substrate extract, adding 0.5% of glucose, 100 mg/100 mL of potassium dihydrogen phosphate, 100 mg/100 mL of dipotassium hydrogen phosphate trihydrate, adjusting the pH value of the material solution, after autoclaving, a fermentation substrate for subsequent microbial fermentation is obtained;
• C. inoculation and fermentation:
• aseptically inoculating two cultures of pre-cultured Bifidobacterium genus into the sterile fermentation substrate obtained in step B, facultative anaerobic culture at 38±1° C. for 2 to 6 hours, inoculating at least one culture of pre-cultured Lactobacillus and continuing fermentation, when a pH value of a fermentation broth drops to 4.1, reducing the temperature to 25° C. and maintaining for another 24 hours, and ending the fermentation;
• D. inactivation:
• inactivating the fermentation broth that is completely fermented at 70° C. or 115° C. to obtain the microbial fermentation composition.