Single-injection methods and formulations to induce and control multiple ovarian follicles in bovine, caprine, ovine, camelid and other female animals
AssigneeTherio, LLC
InventorJohn L. Curtis
Life of this patent
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Abstract
Methods and formulations for a simplified, single-injection method to induce and control the synchronous growth (superstimulation), and ovulation (superovulation) of multiple ovarian follicles in bovine, ovine, caprine, camelid and other female animals enabling the subsequent collection of (a) multiple oocytes when conducting in-vitro fertilization, or (b) multiple embryos when conducting multiple ovulation embryo transfer.
Claims16
A method of inducing ovarian follicular hyperstimulation and superovulation in bovine-animals, the method comprising the steps of:
- administering an in-situ depot formulation comprising a protein microsphere matrix having a diameter ranging from 50-100 microns to a bovine animal, the protein microsphere matrix including controlled release agents, the controlled release agents including a single dose of follicle stimulating hormone (FSH) released over 96 hours beginning 48 hours after administration, and two doses of luteinizing hormone (LH), wherein the first dose of LH provides an immediate surge of LH and the second dose provides at least one calculated LH release (burst) after 144 hours from administration of the protein microsphere matrix,
- wherein the method consists of a single administration of said protein microsphere matrix to the bovine animal,
- wherein the protein microsphere matrix induces ovarian dominant follicle removal, hyperstimulation of follicular growth, superovulation and timed synchronous ovulation of the matured follicle cohort in the bovine animal,
- wherein the protein microsphere matrix comprises at least one polymer, wherein the at least one polymer is selected from a group consisting of polyethylene glycol (PEG), poly(lactic acid) (PLA) or poly(lactide-co-glycolide) (PLGA),
- wherein the protein microsphere matrix is administered via injection.
A method of inducing ovarian follicular hyperstimulation for ovum pickup (OPU) and superovulation for multiple ovulation embryo transfer (MOET) in bovine-animals, the method comprising the steps of:
- administering an in-situ thermal gelling depot formulation which contains a protein microsphere matrix having a diameter ranging from 50-100 microns to a bovine animal, the protein microsphere matrix including controlled release agents, the controlled release agents including a single dose of follicle stimulating hormone (FSH) which is released over 96 hours beginning 48 hours after administration, and two doses of luteinizing hormone (LH), wherein the first dose of LH provides an immediate surge of LH and the second dose provides at least one calculated LH release (burst) after 144 hours from administration of the protein microsphere matrix,
- wherein the method consists of a single administration of said protein microsphere matrix to the bovine animal,
- wherein the protein microsphere matrix induces ovarian dominant follicle removal, hyperstimulation of follicular growth, superovulation and timed synchronous ovulation of the matured follicle cohort in the bovine animal,
- wherein the protein microsphere matrix comprises at least one polymer, wherein the at least one polymer is selected from a group consisting of polyethylene glycol (PEG), poly(lactic acid) (PLA) or poly(lactide-co-glycolide) (PLGA),
- wherein the protein microsphere matrix is administered via injection.
Dependencies are inferred from the claim text.