Methods and kits for using blocked 2-AA for glycan analysis
OwnerSergey Vlasenko, Francis T. Haxo +1inventors
InventorsSergey Vlasenko, Francis T. Haxo, Andres Guerrero Navarro
Life of this patent
Estimating the term…
Abstract
The present disclosure provides methods for using anthranilic acid (2-AA) blocked by tertbutyloxycarbonyl (Boc) groups in protocols for labeling glycans for analysis, and kits providing 2-AA-Boc and acids for unblocking blocked 2-AA for use in glycan labeling and analysis.
Claims33
A method for labeling a glycan with anthranilic acid (“2-AA”), said method comprising (a) obtaining 2-AA conjugated to a tert-butyloxycarbonyl (“Boc”) group (“2-AA-Boc”),
- (b) removing said Boc group by incubating said 2-AA-Boc with an acid for a time and at a temperature sufficient to remove said Boc group from said 2-AA-Boc, thereby obtaining 2-AA, and
- (c) incubating a solution containing said 2-AA and said glycan under conditions that allow said 2-AA to label said glycan, thereby labeling said glycan with 2-AA.
A kit for labeling glycans with anthranilic acid (“2-AA”) by reductive amination, said kit comprising (a) a container containing 2-AA conjugated to a tert-butyloxycarbonyl (“Boc”) group (“2-AA-Boc”), and (b) a container containing an acid suitable for removing said Boc group from said 2-AA-Boc in a concentration sufficient to remove said Boc group from said 2-AA-Boc.
Dependencies are inferred from the claim text.