Construction of a quadruple enterotoxin-deficient mutant of Bacillus thuringiensis
AssigneeWisconsin Alumni Research Foundation
InventorsJo Emily Handelsman, Amy Klimowicz, Changhui Guan
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Abstract
Some HBL and NHE enterotoxins are known to cause food-borne diseases in humans. Enterotoxin-deficient mutants of member strains of the Bacillus cereus group that do not produce HBL, HBLa1, HBLa2, or NHE enterotoxins are disclosed. Enterotoxin-deficient mutants are suitable for use as biocontrol agents. Methods for making the mutants and for using the mutants are described.
Claims20
An isolated mutant Bacillus strain that does not produce functional enterotoxins non-hemolytic enterotoxin (NHE), haemolysin BL (HBL), HBLa1, and HBLa2 and comprises a mutation to an enterotoxin-encoding operon selected from the group consisting of nhe, hbl, hbla1, and hbla2 such that the operon does not encode a functional enterotoxin, wherein the Bacillus is selected from the group consisting of B. cereus, B. thuringiensis, and B. weihenstephanensis.
Dependencies are inferred from the claim text.