Glycan analysis method, glycan analysis system, program for glycan analysis, and kit for glycan analysis

A glycan analysis method, comprising:

• a) a fluorescent-labeling step in which a glycan in a sample is fluorescently labeled;
• b) a measurement step in which a sample containing a fluorescently-labeled glycan is separated by a microchip electrophoretic method using at least two kinds of buffer solutions for separation selected from a simple buffer solution with no lectin added and a plurality of kinds of lectin-added buffer solutions each of which contains a different kind of lectin added to the simple buffer solution, and the sample is fluorescently detected;
• and
• c) an identification step in which the glycan in the sample is identified by comparing a plurality of electropherograms obtained by a measurement using the at least two kinds of different buffer solutions for separation,
• wherein separation conditions in the microchip electrophoresis method including a kind of simple buffer solution are such conditions under which glycans having different degrees of polymerization ranging from monosaccharide to icosasaccharide can be separated from each other in a measurement of an isomalto-oligosaccharide mixture, and
• wherein DSA (Datura stramonium Agglutinin) is used as the lectin.