Processive enzyme molecular electronic sensors for DNA data storage
AssigneeRoswell Biotechnologies, Inc.
InventorsBarry Merriman, Tim Geiser, Paul Mola, Gina Costa
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Abstract
A processive enzyme molecular sensor for use in a DNA data storage system is disclosed that can extract digital information suitably encoded into a synthetic DNA molecule. In various aspects, such sensors are provided in a high-density chip-based format that can provide the high throughput, low-cost and fast data extraction capability required for large scale DNA data storage systems. The sensor for reading the digital data stored in DNA molecules processes individual encoded DNA molecules directly, eliminating the need for complicated sample preparation such as making copies of DNA or clonal populations of such molecules.
Claims10
A sensor comprising:
- a first electrode;
- a second electrode spaced apart from the first electrode by an electrode gap;
- a gate electrode capacitively coupled to the electrode gap;
- a DNA bridge molecule spanning the electrode gap and having a first end and a second end, the first end conjugated to the first electrode and the second end conjugated to the second electrode;
- a processive enzyme conjugated to the first electrode and the second electrode via the DNA bridge molecule, the processive enzyme comprising a native or genetically engineered polymerase, a reverse transcriptase, a helicase, an exonuclease, or a molecular motor for packaging of viral DNA;
- and
- a trans-impedance amplifier electrically connected to the gate electrode and at least one of the first electrode and the second electrode, the trans-impedance amplifier providing a biasable voltage to the gate electrode and an output comprising a measurable electrical parameter.
A sensor comprising:
- a first electrode;
- a second electrode spaced apart from the first electrode by an electrode gap;
- a gate electrode capacitively coupled to the electrode gap;
- at least two arm molecules electrically connected a processive enzyme to the first electrode and the second electrode, wherein one of the at least two arm molecules is selected from a double stranded DNA and a protein alpha helix, and the processive enzyme comprises a native or genetically engineered polymerase, a reverse transcriptase, a helicase, an exonuclease, or a molecular motor for packaging of viral DNA;
- and
- a trans-impedance amplifier electrically connected to the gate electrode and at least one of the first electrode and the second electrode, the trans-impedance amplifier providing a biasable voltage to the gate electrode and an output comprising a measurable electrical parameter.
Dependencies are inferred from the claim text.