Method for preparation of liquid mixtures
AssigneeCytiva Sweden AB
InventorsEnrique Napoleon Carredano, Elenor Strandberg
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Abstract
The present invention relates to the preparation of liquid mixtures, and more particularly to the preparation of a liquid mixture, such as a buffer, wherein the conductivity of the liquid mixture is measured and the pH indirectly determined if the buffer concentration is known. Another object of the present invention is to provide a method of preparing a liquid mixture with a predetermined pH value by using conductivity as feedback control parameter.
Claims14
A buffer system comprising,
- a. a conductivity sensor for measuring conductivity of a buffer;
- b. a concentration sensor for measuring or calculating concentration of the buffer;
- and
- c. means for adding controlled amounts of buffer components to said buffer based on the measured conductivity and the measured or calculated concentration of the buffer to maintain buffer concentration constant until a specific conductivity value is obtained,
- wherein said specific conductivity value is correlated to a predetermined pH value and the buffer concentration by a surface model trained using a training data set with known concentrations, specific conductivities and pHs obtained from a training set of buffer systems and based at least in part on measuring the pH for the solutions belonging to the training data set and using a numerical regression method to obtain the surface model equation as a regression model from the training data.
A device comprising a computer and memory storing a computer program product comprising instructions that, when executed by the computer, causes the computer to perform a method of preparing a buffer having a predetermined pH value, the method comprising,
- a. measuring conductivity of a buffer using a conductivity sensor;
- b. measuring or calculating concentration of the buffer;
- and
- c. adding controlled amounts of buffer components to said buffer based on the measured conductivity and the measured or calculated concentration of the buffer to maintain buffer concentration constant until a specific conductivity value is obtained,
- wherein said specific conductivity value is correlated to a predetermined pH value and the buffer concentration by a surface model, comprising using a training data set with known concentrations, specific conductivities and pHs obtained from a training set of buffer systems;
- and measuring the pH for the solutions belonging to the training data set and using a numerical regression method to obtain the surface model equation as a regression model from the training data.
Dependencies are inferred from the claim text.