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US 11,371,064 B2Granted
Transformed yeast producing novel 1-octen-3-ol, and preparation method therefor
AssigneesKyongsangbuk-Do, Kyungpook National University Industry-Academic Cooperation Foundation
InventorsWoo Jae Cheon, Kwang Seon Lee, Sang Wung Kim, Jong-Guk Kim, Nan Yeong Lee, Minji Jeong
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Abstract
The present application relates to a method for preparing transformed yeast producing 1-octen-3-ol, and yeast prepared by the method, and is useful in the cosmetic industry and the food development industry which use a Tricholoma matsutake scent.
Claims7
A transformed yeast for producing 1-octen-3-ol transformed with a recombinant vector comprising
- the nucleotide sequence of any of SEQ ID NOs:
- 9, 10 and 11 encoding lipoxygenase and the nucleotide sequence of SEQ ID NO:
- 12 encoding hydroperoxide lyase.
A method for producing a transformed yeast for producing 1-octen-3-ol comprising the steps of:
- isolating total RNA of pine mushroom and synthesizing cDNA;
- PCR-amplifying a lipoxygenase gene comprising the nucleotide sequence of any of SEQ ID NOs:
- 9-11 and a hydroperoxide lyase gene comprising the nucleotide sequence of SEQ ID NO:
- 12 from the synthesized cDNA;
- gene-cloning each of the amplified lipoxygenase gene and hydroperoxide lyase gene in a vector;
- gene-cloning each of the cloned lipoxygenase gene and hydroperoxide lyase gene in each yeast expression vector;
- and
- transforming and incubating the yeast expression vector into a yeast to produce 1-octen-3-ol.
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