Increasing plant growth and yield by expression of an m-type thioredoxin
AssigneeBenson Hill, Inc.
InventorsBenjamin Neil Gray, Henry D. Priest
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Abstract
Compositions and methods for improving plant growth are provided herein. Disclosed herein are polypeptides encompassing m-type thioredoxin proteins, and polynucleotides encoding m-type thioredoxin proteins, wherein the thioredoxin protein-encoding sequence is expressed from mesophyll-preferred promoters. Also provided herein are expression constructs for expressing genes of interest whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing transgenic plants.
Claims11
A method for increasing crop yield comprising transforming a plant with at least one m-type thioredoxin protein-encoding sequence comprising
- an m-type thioredoxin sequence encoding a protein having at least 85% identity to SEQ ID NO:2, wherein the protein is a functional m-type thioredoxin protein,
- wherein said m-type thioredoxin protein-encoding sequence is operably linked to a promoter comprising SEQ ID NO:7.
A DNA construct comprising, in operable linkage,
- a) a promoter comprising SEQ ID NO:7 and,
- b) an m-type thioredoxin protein-encoding sequence that comprises SEQ ID NO:1 or
- encodes a protein selected from the group consisting of SEQ ID NOs:2 and 17-20, wherein said promoter is heterologous to said m-type thioredoxin protein-encoding sequence.
A DNA construct comprising, in operable linkage,
- a) a promoter comprising SEQ ID NO:7 and,
- b) an m-type thioredoxin protein-encoding sequence that encodes a protein
- comprising an amino acid sequence having at least 85% sequence identity to SEQ ID
- NO:2, wherein the protein is a functional m-type thioredoxin protein, and wherein said promoter is heterologous to said m-type thioredoxin protein-encoding sequence.
Dependencies are inferred from the claim text.