Systems and methods for manufacturing a microfluidic rotor device

A method, comprising:

• manufacturing a microfluidic rotor device by bonding a first layer and a second layer using two-shot injection molding, wherein the first layer coupled to the second layer collectively defines a set of wells, the first layer being substantially transparent, and the second layer defining a channel, the second layer being substantially absorbent to infrared radiation;
• disposing a lyophilized reagent in one or more wells of the set of wells;
• aligning the second layer to a third layer after the bonding of the first layer and the second layer;
• aligning a photomask to the third layer after the bonding of the first layer and the second layer, the photomask configured to block the infrared radiation to one or more portions of the second layer;
• and
• bonding the third layer to the second layer using infrared radiation via an infrared laser beam, the photomask configured to block the infrared laser beam over the lyophilized reagent, and the third layer defining an opening configured to receive a fluid, the third layer being substantially transparent, wherein the channel establishes a fluid communication path between the opening and the set of wells.