Pharmaceutical composition containing a stabilised mRNA optimised for translation in its coding regions
AssigneeCureVac AG
InventorsFlorian Von Der Mülbe, Ingmar Hoerr, Steve Pascolo
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Abstract
The present invention relates to a pharmaceutical composition comprising a modified mRNA that is stabilised by sequence modifications and optimised for translation. The pharmaceutical composition according to the invention is particularly well suited for use as an inoculating agent, as well as a therapeutic agent for tissue regeneration. In addition, a process is described for determining sequence modifications that promote stabilisation and translational efficiency of modified mRNA of the invention.
Claims19
A method of expressing a polypeptide in a subject comprising administering an effective amount of a pharmaceutical composition comprising a mRNA encoding the polypeptide to the subject, wherein the mRNA encoding polypeptide comprises at least one nucleotide of the mRNA that is substituted with an analog of a naturally occurring nucleotide, wherein the polypeptide encoded by the mRNA is a therapeutic polypeptide, which is not an infectious disease antigen or a tumor antigen, and wherein the mRNA that encodes the polypeptide comprises a coding sequence encoding the polypeptide with an increased G/C content of at least 7 percentage points relative to the coding sequence of an original RNA sequence encoding the polypeptide.
Dependencies are inferred from the claim text.