Method of generating plants having white foliage
AssigneeKlemm+Sohn GmbH & Co. KG
InventorsVinicius Vilperte, Thomas Debener, Robert Boehm, Andrea Dohm, Guido Von Tubeuf, Ulrich Sander
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Abstract
The disclosure relates to a method for the generation of plants, such as Euphorbia pulcherrima, having a dysfunctional glutathione S-transferase (GST), and the seeds, plant parts or plant cells derived therefrom. The disclosure further relates to a molecular marker capable of identifying a dysfunctional GST gene, to isolated DNA encoding such a dysfunctional GST gene and to the use of such DNA for the preparation of a molecular marker and for use in methods of targeted mutagenesis to inactivate the GST gene to generate plants with a white foliage phenotype.
Claims10
A method for the generation of a Euphorbia pulcherrima plant having a white foliage phenotype comprising the steps of:
- a) selecting a target E. pulcherrima plant which comprises in its genome at least one dysfunctional allele of a glutathione S-transferase gene (EpGST) comprising a CTTC deletion in a simple sequence repeat (SSR) in the region encoding amino acids at positions 40-50 of the functional EpGST protein of SEQ ID NO:
- 3, comprising a stretch of 12 nucleotides consisting of a threefold CTTC repeat, wherein the selecting comprises using a molecular marker suitable to detect the presence or absence of a CTTC deletion in said SSR motif of the EpGST gene;
- b) subjecting the target E. pulcherrima plant to a mutagenesis treatment to produce a mutant E. pulcherrima plant;
- c) selecting a white foliaged E. pulcherrima plant homozygous for said CTTC deletion in said SSR motif.
A method for the generation of a Euphorbia pulcherrima plant having a white foliage phenotype comprising the steps of:
- a) providing a target E. pulcherrima plant without a white foliage phenotype comprising in its genome at least one functional allele of a glutathione S-transferase gene (EpGST) comprising a simple sequence repeat (SSR) in the region encoding amino acids at positions 40-50 of the protein of SEQ ID NO:
- 3, comprising a stretch of 12 nucleotides consisting of a threefold CTTC repeat;
- b) subjecting said E. pulcherrima plant to a mutagenesis treatment to produce a mutant E. pulcherrima plant;
- c) selecting a mutant E. pulcherrima plant, wherein at least one allele of the EpGST gene comprises a CTTC deletion in said SSR motif, wherein the selecting comprises using a molecular marker suitable for the detection of said CTTC deletion in said SSR motif of the EpGST gene;
- d) repeating steps b) and c) until all alleles of the EpGST gene in the plant genome comprise said CTTC deletion in said SSR motif;
- and
- e) selecting a white foliaged E. pulcherrima plant homozygous for said CTTC deletion in said SSR motif.
Dependencies are inferred from the claim text.