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US 11,365,418 B2Granted
Dual cistronic bacterial expression system
AssigneeLupin Limited
InventorsShardul Salunkhe, Brajesh Varshney, Sudheerbabu Soorapaneni
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Abstract
The present invention relates to the dual, independent cistron expression system in a single vector for the production of protein of interest proteins and peptides expressed as insoluble inclusion bodies formed in the bacteria E. coli. The present invention also provides the process for the expression of protein of interest using said bicistronic vector.
Claims13
A dual cistronic expression vector for expression in a prokaryotic host cell, comprising:
- a) a first cistron, comprising a T7 promoter having the sequence of SEQ ID NO:
- 10 operably linked to a polynucleotide sequence encoding a heavy chain of an antibody or a fragment thereof, where the fragment thereof is a Fab fragment;
- b) a second cistron, comprising an arabinose promoter having the sequence of SEQ ID NO:
- 14 operably linked to a polynucleotide sequence encoding a light chain of an antibody or a fragment thereof, where the fragment thereof is a Fab fragment;
- wherein, the first and second cistrons are positioned within the same vector and express both the heavy chain and the light chain proteins at equimolar levels in the form of inclusion bodies.
2 more elements
Dependencies are inferred from the claim text.