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US 11,365,403 B2Granted
Beta-lactamase variants
AssigneesDa Volterra, Bioaster
InventorsJean De Gunzburg, Jean-Denis Docquier
Life of this patent
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Abstract
The present invention relates to an isolated polypeptide having beta-lactamase activity and nucleic acid sequences encoding the polypeptide. The isolated polypeptide of the invention is a VIM-2 variant with improved properties such as improved protease stability, stability in intestinal medium, improved activity against one or more antibiotics, improved specific activity and/or improved production in a host cell.
Claims16
A polypeptide having beta-lactamase activity, which comprises an amino acid sequence having at least 90% sequence identity to SEQ ID NO:1, said polypeptide comprising:
- a substitution at each of positions 10 and 22;
- a substitution at each of positions 10 and 34;
- a substitution at each of positions 10 and 130;
- a substitution at each of positions 10, 22, and 34;
- a substitution at each of positions 10, 22, and 130;
- a substitution at each of positions 10, 34, and 130;
- or
- a substitution at each of positions 10, 22, 34, and 130,
- wherein the positions correspond to the positions in the sequence of SEQ ID NO:1, the substitution at position 10 is V10A, the substitution at position 22 is Q22H or Q22N, the substitution at position 34 is Q34R, the substitution at position 130 is E130D, and said polypeptide has improved stability and beta-lactamase activity as compared with wild-type VIM-2 of SEQ ID NO:1.
6 more elements
Dependencies are inferred from the claim text.