Increasing plant growth and yield by using a glutaredoxin
AssigneeBenson Hill, Inc.
InventorBenjamin Neil Gray
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Abstract
Compositions and methods for improving plant growth are provided herein. Polynucleotides encoding glutaredoxin proteins, polypeptides encompassing glutaredoxin proteins, and expression constructs for expressing genes of interest whose expression may improve agronomic properties including but not limited to crop yield, biotic and abiotic stress tolerance, and early vigor, plants comprising the polynucleotides, polypeptides, and expression constructs, and methods of producing transgenic plants are also provided.
Claims12
A method for increasing crop yield comprising transforming a plant with at least one glutaredoxin protein-encoding sequence;
- wherein the glutaredoxin protein-encoding sequence encodes a protein which shares at least 80% sequence identity with SEQ ID NO 3;
- wherein the glutaredoxin protein-encoding sequence is operably linked to a heterologous bundle sheath cell-preferred promoter which comprises SEQ ID NO 10.
A plant having stably incorporated into its genome a promoter that drives expression in a plant operably linked to a glutaredoxin protein-encoding sequence, wherein said promoter is heterologous to said glutaredoxin protein-encoding sequence;
- wherein the glutaredoxin protein-encoding sequence encodes a protein which shares at least 80% sequence identity with SEQ ID NO:
- 3;
- wherein the heterologous bundle sheath cell-preferred promoter comprises SEQ ID NO:
- 10.
A DNA construct comprising, in operable linkage,
- a. A promoter that is functional in a plant cell, wherein the said promoter is a bundle sheath cell-preferred promoter which comprises SEQ ID NO:
- 10;
- and,
- b. A nucleic acid sequence encoding a glutaredoxin protein which shares at least 80% sequence identity with SEQ ID NO:
- 3.
Dependencies are inferred from the claim text.