Back to results
US 11,353,466 B2Granted
Methods and compositions for quantifying hemoglobin
AssigneeCellics Therapeutics, Inc.
InventorWeidong Xu
Life of this patent
Estimating the term…
Abstract
The present invention relates to methods and compositions for quantifying hemoglobin using, inter alia, a peroxidase substrate and hydrogen peroxide.
Claims22
A method for quantifying hemoglobin in a sample, which method comprises:
- a) contacting a sample containing or suspected of containing hemoglobin with a peroxidase substrate and hydrogen peroxide, under suitable conditions, for allowing oxidation of said peroxidase substrate to form an oxidation product;
- and b) assessing said peroxidase substrate and/or said oxidation product to quantify hemoglobin in said sample, wherein the sample comprises a cellular membrane coated nanoparticle, said nanoparticle comprising an inner core comprising a non-cellular material and an outer surface comprising a cellular membrane derived from a hemoglobin-containing cell.
A kit for quantifying hemoglobin in a sample, which kit comprises:
- a) a peroxidase substrate;
- b) hydrogen peroxide;
- and c) a solution or suspension that has basic pH and/or contains a surfactant for preparing a sample, wherein the sample comprises a cellular membrane coated nanoparticle, said nanoparticle comprising an inner core comprising a non-cellular material and an outer surface comprising a cellular membrane derived from a hemoglobin-containing cell.
Dependencies are inferred from the claim text.