Back to results
US 11,352,648 B2Granted
Metabolic engineering for microbial production of terpenoid products
AssigneeManus Bio Inc.
InventorsAjikumar Parayil Kumaran, Ryan Lim, Jason Donald, Hsien-Chung Tseng, Christine Nicole S. Santos, Ryan Philippe
Life of this patent
Estimating the term…
Abstract
The invention relates to methods and bacterial strains for making terpene and terpenoid products, the bacterial strains having improved carbon pull through the MEP pathway and to a downstream recombinant synthesis pathway.
Claims33
A method for production of a terpenoid product, comprising:
- providing a bacterial strain expressing a terpenoid biosynthesis pathway, the terpenoid biosynthesis pathway comprising an upstream methylerythritol phosphate pathway (MEP pathway) and a recombinant downstream synthesis pathway, wherein the bacterial strain overexpresses IspG and IspH and one or more recombinant oxidoreductase enzymes enhancing supply and/or transfer of electrons to the IspG and IspH;
- and
- culturing the bacterial strain to produce the terpene or terpenoid product,
- wherein said one or more recombinant oxidoreductase is a pyruvate:flavodoxin oxidoreductase (PFOR).
1 more element
Dependencies are inferred from the claim text.