Rapid fluorescence tagging of glycans and other biomolecules with enhanced MS signals
AssigneeWaters Technologies Corporation
InventorsDarryl W. Brousmiche, Matthew A. Lauber
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Abstract
Reagents comprising MS active, fluorescent molecules with an activated functionality for reaction with amines useful in tagging biomolecules such as N-glycans and uses thereof are taught and described. In particular embodiments, the MS active, fluorescent molecules are of the compound of the Formula III or of the Formula IV where R1, R2 and R3 are defined herein.
Claims7
A compound of the structural Formula III:
- wherein
- R1 is
- R2 is
- R2a is selected from ester, amide, amine, ether, urea, carbamate, carbonate, thiol, thiourea, thiocarbamate, alkyl or carbonyl;
- R2b is
- x=0-12;
- y=0-12;
- z=1-12;
- and
- salts or solvates thereof, wherein said compound is a mass spectrometry (MS) active, fluorescent compound.
A compound of the structural Formula IV:
- wherein
- R1 is O or N;
- R2 is
- R3 is
- R3a is selected from ester, amide, amine, ether, urea, carbamate, carbonate, thiol, thiourea, thiocarbamate, alkyl or carbonyl;
- R3b is
- x=0-12;
- y=0-12;
- z=1-12;
- and
- salts or solvates thereof.
A compound of Formula IV:
- wherein
- FL is
- R1 is O or N;
- R2 is
- R3 is
- R3a is selected from ester, amide, amine, ether, urea, carbamate, carbonate, thiol, thiourea, thiocarbamate, alkyl or carbonyl;
- R3b is
- x=0-12;
- y=0-12;
- z=1-12;
- and salts or solvates thereof, wherein said compound is a mass spectrometry (MS) active, fluorescent compound.
A method for analyzing a glycan in a sample containing at least one glycan by means of liquid chromatography and mass spectrometry comprising labeling the glycan in the sample by reacting the glycan with a compound according to Formula III for a time and under conditions suitable to facilitate the labeling;
- providing a sample containing the glycan labeled with the compound;
- and subjecting the labeled compound to liquid chromatograph and mass spectrometry wherein the compound of Formula III is:
- wherein
- R1 is
- R2 is
- R2a is selected from ester, amide, amine, ether, urea, carbamate, carbonate, thiol, thiourea, thiocarbamate, alkyl or carbonyl;
- R2b is
- x=0-12;
- y=0-12;
- z=1-12;
- salts or solvates thereof.
Dependencies are inferred from the claim text.