Stable gene transfer to proliferating cells

A method for treating a disease of, affecting, or associated with, proliferating liver cells, comprising administering to a subject in need thereof (i) a recombinant AAV (rAAV) vector comprising a transgene operably linked to a liver-specific promoter and flanked by piggyBac transposon-derived inverted terminal repeat sequences, which sequences are in turn flanked by AAV-derived inverted terminal repeat regions;

• and (ii) a vector encoding a piggyBac transposase and providing transient expression of the transposase, wherein the transposase recognises said piggyBac transposon-derived inverted terminal repeat sequences and directs the genomic integration of the transgene into the genome of the proliferating liver cells, wherein said administration results in the stable integration and expression of the transgene in the proliferating liver cells to thereby treat the disease, and wherein the disease is associated with the deficiency of one or more gene products in the proliferating liver cells, and wherein expression of the transgene normalizes production and activity of the deficient Rene product.