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US 11,345,949 B2Granted
Methylated control DNA
AssigneeExact Sciences Corporation
InventorsHatim T. Allawi, Graham P. Lidgard
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Abstract
Provided herein is technology relating compositions and methods for analysis of methylated DNA from a subject. The technology also relates to use of endogenous methylated DNAs as internal controls for marker gene methylation assays.
Claims10
A composition, comprising:
- a) a bisulfite-converted B3GALT6 DNA comprising SEQ ID NO:2 or the full complement of SEQ ID NO:2, and
- b) a pair of primers complementary to primer binding sites within SEQ ID NO:2 or the complement thereof.
A method, the method comprising:
- a) treating DNA from a sample with a bisulfite reagent to produce a bisulfite-converted B3GALT6 DNA;
- b) amplifying a region of said bisulfite-converted DNA using a pair of nucleic acid primers;
- and
- c) detecting an amplified B3GALT6 DNA comprising SEQ ID NO:2 or the full complement thereof.
1 more element
Dependencies are inferred from the claim text.