Method and pharmaceutical composition for continuously maintaining growth of a motor neuron progenitor cell

A method for continuously maintaining growth of a motor neuron progenitor cell, comprising:

• culturing a motor neuron progenitor cell under a plurality of culture mediums sequentially to make the motor neuron progenitor cell maintain the ability to self-renew and differentiate to a mature motor neuron cell,
• wherein the plurality of culture mediums comprising a first culture medium, a second culture medium, a third culture medium and a fourth culture medium, the first culture medium contains 10% knockout serum replacement, the second culture medium contains knockout serum replacement and retinoic acid, the third culture medium is neurobasal media containing retinoic acid and purmorphamine for co-culturing, the fourth culture medium is neurobasal media,
• wherein the motor neuron progenitor cell is cultured in the first culture medium on the first to the third day, in the second medium on the third to fifth day, in the third medium on the fifth to seventh days under a low-density condition of 100 cells/mL, and in the fourth culture medium until the eighth day,
• wherein the motor neuron progenitor cell is cultured in the fourth culture medium pre-treated with the olfactory ensheathing cell under high density condition of 10000 cells/ml and then the olfactory ensheathing cell is removed.