Fluidic device for aliquoting and combinatorial mixing of liquids

A microfluidic fluidic device for aliquoting and pairwise combinatorial mixing of at least three first liquid samples with at least three second liquid samples, the microfluidic device comprising:

• six reservoirs comprising a first reservoir for each of the three first liquid samples and a second reservoir for each of the three second liquid samples;
• eighteen aliquot chambers comprising:
• three first aliquot chambers fluidically connected to each of the first reservoirs by first microfluidic conduits having a first orientation relative to the device;
• three second aliquot chambers fluidically connected to each of the second reservoirs by second microfluidic conduits having a first orientation relative to the device;
• nine mixing chambers, in which each mixing chamber is fluidically connected to at least one of the first aliquot chambers and at least one of the second aliquot chambers by third microfluidic conduits having a second orientation relative to the device,
• wherein the first and second microfluidic conduits are configured such that application of a first rotor-driven unidirectional centrifugal force field effects liquid flow in the first orientation to generate three aliquots of each of the three first liquid samples in the first aliquot chambers and three aliquots of each of the three second liquid samples in the second aliquot chambers,
• wherein the third microfluidic conduits are configured such that application of a second rotor-driven unidirectional centrifugal force field effects liquid flow in the second orientation to combinatorially combine aliquots of the three-first liquid samples with aliquots of the three second liquid samples in a pairwise manner,
• wherein the third microfluidic conduits are configured to extend against the direction of the first rotor-driven unidirectional centrifugal force field to prevent movement of liquid from the first aliquot chambers and second aliquot chambers to the mixing chambers during application of the first rotor-driven unidirectional centrifugal force field,
• wherein at least some of the conduits are spatially separated across the Y-axis of the device.

A fluidic device for aliquoting and pairwise combinatorial mixing of at least two first liquid samples with at least two second liquid samples, the microfluidic device comprising:

• a first reservoir for each of the at least two first liquid samples and a second reservoir for each of the at least two second liquid samples;
• at least two first aliquot chambers fluidically connected to each of first reservoirs by first microfluidic conduits having a first orientation relative to the device;
• at least two second aliquot chambers fluidically connected to each of the second reservoirs by second microfluidic conduits having a first orientation relative to the device;
• at least four mixing chambers, in which each mixing chamber is fluidically connected to at least one of the first aliquot chambers and at least one of the second aliquot chambers by third microfluidic conduits having a second orientation relative to the device,
• wherein the first and second microfluidic conduits are configured such that application of a first rotor-driven unidirectional centrifugal force field effects liquid flow in the first orientation to generate aliquots of the at least two first liquid samples in the first aliquot chambers and the at least two second liquid samples in the second aliquot chambers,
• wherein the third microfluidic conduits are configured such that application of a second rotor-driven unidirectional centrifugal force field effects liquid flow in the second orientation to combinatorially combine aliquots of the at least two first liquid samples with aliquots of the at least two second liquid samples in a pairwise manner,
• wherein at least some of the conduits are spatially separated across the Y-axis of the device, and
• wherein the first and second microfluidic conduits are configured to have a higher fluidic resistance than the third microfluidic conduits.

A fluidic device for aliquoting and pairwise combinatorial mixing of at least two first liquid samples with at least two second liquid samples, the microfluidic device comprising:

• a first reservoir for each of the at least two first liquid samples and a second reservoir for each of the at least two second liquid samples;
• at least two first aliquot chambers fluidically connected to each of first reservoirs by first microfluidic conduits having a first orientation relative to the device;
• at least two second aliquot chambers fluidically connected to each of the second reservoirs by second microfluidic conduits having a first orientation relative to the device;
• at least four mixing chambers, in which each mixing chamber is fluidically connected to at least one of the first aliquot chambers and at least one of the second aliquot chambers by third microfluidic conduits having a second orientation relative to the device,
• wherein the first and second microfluidic conduits are configured such that application of a first rotor-driven unidirectional centrifugal force field effects liquid flow in the first orientation to generate aliquots of the at least two first liquid samples in the first aliquot chambers and the at least two second liquid samples in the second aliquot chambers,
• wherein the third microfluidic conduits are configured such that application of a second rotor-driven unidirectional centrifugal force field effects liquid flow in the second orientation to combinatorially combine aliquots of the at least two first liquid samples with aliquots of the at least two second liquid samples in a pairwise manner,
• wherein at least some of the conduits are spatially separated across the Y-axis of the device, and
• including a third reservoir for a third liquid sample, and a conduit configured to deliver an aliquot of the third liquid sample to the at least four mixing chambers.