Methods and compositions for red-shifted chromophore substitution for optogenetic applications
AssigneeWashington University
InventorsJoseph Corbo, Jennifer Enright, Matthew Toomey
Life of this patent
Estimating the term…
Abstract
The present disclosure provides compositions and methods used for optogenetics, wherein the composition comprises an optogenetic device and a retinoid processing enzyme.
Claims13
A method of modulating electrical activity of a cell comprising:
- administering to the cell a composition comprising a vector wherein, the vector comprises a polynucleotide sequence encoding a polypeptide, wherein the polynucleotide is operably linked to a promoter suitable for expression in the cell;
- the polypeptide comprises at least one optogenetic device and a retinol dehydrogenase capable of adding a 3,4-double bond in the beta-ionone ring of a Vitamin A molecule;
- and
- activating the optogenetic device with light comprising red spectrum wavelength light.
A method of red-shifting the activation wavelength of an optogenetic device in a subject, the method comprising administering a composition comprising a vector, the vector comprising a polynucleotide sequence encoding a polypeptide, wherein the polynucleotide is operably linked to a promoter suitable for expression in the cell in the subject, and wherein the polypeptide comprises at least one optogenetic device and a retinol dehydrogenase capable of adding a 3,4-double bond in the beta-ionone ring of a Vitamin A molecule.
Dependencies are inferred from the claim text.