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US 11,340,228 B2Granted
Methods for analyzing rare circulating cells
AssigneeEpic Sciences, Inc.
InventorDena Marrinucci
Life of this patent
Estimating the term…
Abstract
The disclosure provides methods for analyzing rare circulating cells (RCCs) at cellular and molecular level following their detection in non-enriched blood samples, methods of this disclosure serve as diagnostic methods for several disease conditions, including cardiovascular diseases and cancer.
Claims39
A method for analyzing rare circulating cells (RCCs) in a non-enriched blood sample, comprising:
- (a) detecting RCCs in the non-enriched blood sample, comprising i) determining presence or absence of one or more immunofluorescent RCC detection markers in nucleated cells in the non-enriched blood sample, and ii) assessing the morphology of the nucleated cells, wherein RCCs are detected among the nucleated cells based on a combination of distinct immunofluorescent staining and morphological characteristics;
- (b) quenching the immunofluorescence of the one or more immunofluorescent RCC detection markers comprising contacting the RCCs with a quenching buffer, wherein the immunofluorescence is quenched by more than 50%, 60%, 70%, 80%, 90%, 95%, 99%, 99.9% or 99.99%;
- and (c) analyzing the detected RCCs, comprising determining presence or absence of one or more fluorescent RCC analysis markers.
Dependencies are inferred from the claim text.