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US 11,339,412 B2Granted
Metabolic engineering for microbial production of terpenoid products
AssigneeManus Bio Inc.
InventorsAjikumar Parayil Kumaran, Chin-Giaw Lim, Souvik Ghosh, Christopher Pirie, Jason Donald, Aaron Love, Hong Nan, Hsien-Chung Tseng, Christine Nicole S. Santos, Ryan Philippe
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Abstract
In various aspects and embodiments, the invention relates to bacterial strains and methods for making terpene and terpenoid products. The invention provides bacterial strains with improved carbon flux through the MEP pathway, to thereby increase terpene and/or terpenoid product yield by fermentation with carbon sources such as glucose.
Claims18
A bacterial strain comprising:
- a recombinant terpenoid synthesis pathway comprising a terpene synthase, the bacterial strain producing isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) through the methylerythritol phosphate (MEP) pathway, and converting the IPP and DMAPP to a terpene or terpenoid through the recombinant terpenoid synthesis pathway;
- and
- one or more genetic modifications that increase MEP carbon, said genetic modifications comprising
- a ryhB deletion or inactivation.
1 more element
Dependencies are inferred from the claim text.