Nucleotide sequence encoding 9-lipoxygenase and recombinant constructs comprising the same

A process for synthesis of recombinant 9 lipoxygenase, the process comprising:

• (a) synthesizing polynucleotide having the nucleotide sequence as set forth in SEQ ID NO:
• 1 with primers having a sequence selected from the group consisting of SEQ ID NO:
• 4, SEQ ID NO:
• 5, SEQ ID NO:
• 6, and SEQ ID NO:
• 7;
• (b) cloning the polynucleotide having the nucleotide sequence as set forth in SEQ ID NO:
• 1 obtained in (a) into a plasmid expression vector using expression vector specific primers to obtain a recombinant plasmid expression vector;
• (c) transforming the recombinant plasmid expression vector obtained in (b) into a host cell to obtain a transformed host cell;
• (d) culturing the transformed host cell obtained in (c) at a temperature from 12° C. to 25° C. in a culture medium;
• (e) separating the recombinant host cells from the culture medium;
• and
• (f) performing lysis and sonication of recombinant host cells to isolate the recombinant 9 lipoxygenase.