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US 11,339,377 B2Granted
SO3 chromatography for use in a method for virus purification
AssigneeBluesky Immunotherapies GmbH
InventorsMarkus Wolschek, Manfred Reiter, Ales Strancar, Mojca Tajnik Sbaizero
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Abstract
The present invention refers to a process for purifying virus particles from cell culture, comprising the steps of subjecting the cell culture to centrifugation to get a supernatant fraction of virus particles, incubating said fraction with a nuclease, diluting the fraction with low conductivity buffer, subjecting said diluted fraction to a SO3 chromatography step, performing a washing step with low conductivity buffer, and eluting virus particles.
Claims19
A process for purifying virus particles from cell culture, comprising the steps in the following order:
- a) subjecting the cell culture to centrifugation to obtain a supernatant fraction of virus particles,
- b) incubating the supernatant fraction with a nuclease,
- c) diluting the supernatant fraction with a low conductivity dilution buffer to obtain a diluted fraction,
- d) subjecting the diluted fraction to a SO3 chromatography step,
- e) performing a washing step with low conductivity wash buffer, wherein the wash buffer has a conductivity of 12 mS/cm or less, and
- f) eluting virus particles.
3 more elements
Dependencies are inferred from the claim text.