Stable production of virulent enterovirus 71 and use thereof
AssigneeTokyo Metropolitan Institute of Medical Science
InventorsSatoshi Koike, Kyousuke Kobayashi, Yui Sudaka, Ayumi Imura
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Abstract
Provided is a host cell for stably propagating a virulent hand, foot and mouth disease virus, the host cell expressing no heparan sulfate and overexpressing primate scavenger receptor class B member 2 (SCARB2). Also provided is a method for screening for an anti-hand, foot and mouth disease virus vaccine or an anti-hand, foot and mouth disease virus drug using a stably cultured virulent hand, foot and mouth disease virus.
Claims8
A host cell for stably propagating a virulent hand, foot and mouth disease virus, wherein the host cell is a mammalian cell, wherein the virus is enterovirus 71. Coxsackie virus A16, Coxsackie virus A14 or Coxsackie virus A7, the host cell expressing no heparan sulfate due to knock-out or knock-down of at least one gene encoding an enzyme involved in the biosynthesis of heparan sulfate in the host cell, and overexpressing primate scavenger receptor class B member 2 (SCARB2) beyond the expression level of a host cell harboring no exogenous SCARB2-encoding gene.
Dependencies are inferred from the claim text.