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US 11,333,662 B2Granted
Potency assay of secretomes
AssigneeAposcience AG
InventorsHendrik Jan Ankersmit, Michael Mildner
Life of this patent
Estimating the term…
Abstract
The present invention relates to a method for determining the potency of a supernatant of a mammalian cell culture to be used in the treatment of an inflammatory condition.
Claims20
A method for determining potential of a supernatant of a mammalian cell culture to be used in treatment of an inflammatory condition comprising steps of
- a) incubating SH-SY5Y cells in a culture medium comprising or consisting of said supernatant,
- b) measuring a promoter activity of an activator protein 1 (AP-1) promoter, wherein the supernatant of the mammalian cell culture has the potential to be used in the treatment of the inflammatory condition, if the promoter activity of the AP-1 promoter is at least 50% higher compared to the AP-1 promoter activity measured when the SH-SY5Y cells are cultivated in a culture medium lacking said supernatant.
A method for determining potential of a supernatant of a mammalian cell culture to be used in treatment of an inflammatory condition comprising steps of
- a) incubating SH-SY5Y cells in a culture medium comprising or consisting of said supernatant,
- b) measuring a promoter activity of an activator protein 1 (AP-1) promoter, wherein the supernatant of the mammalian cell culture has the potential to be used in the treatment of the inflammatory condition, if the promoter activity of the AP-1 promoter is up to 10% higher or lower compared to the promoter activity measured when the SH-SY5Y cells are cultivated in a culture medium comprising a reference supernatant of mammalian cells known to be effective for the treatment of the inflammatory condition.
Dependencies are inferred from the claim text.