Nucleic acids and recombinant host cells expressing acetylserotonin O-methyltransferase (ASMT) variants and their use in producing melatonin
AssigneeDanmarks Tekniske Universitet
InventorHao Luo
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Abstract
Described herein are variants of acetylserotonin O-methyltransferase (ASMT) as well as vectors and recombinant microbial host cells expressing such ASMT variant and their use in producing melatonin and related compounds. Preferred ASMT variants provide for a higher turnover of N-acetylserotonin into melatonin.
Claims19
A nucleic acid encoding a variant of Homo sapiens acetylserotonin O-methyltransferase (ASMT), the variant having at least 98% sequence identity to the polypeptide of SEQ ID NO:
- 1 and comprising one or more mutations in the segment corresponding to residues 258 to 272 in the polypeptide of SEQ ID NO:
- 1, wherein the variant has an increased catalytic activity in converting N-acetylserotonin to melatonin as compared to the polypeptide of SEQ ID NO:
- 1 and comprises a mutation in one or more residues corresponding to residues 258, 260, or 272 in the polypeptide of SEQ ID NO:
- 1.
A nucleic acid encoding a variant of Homo sapiens ASMT, the variant having at least 92% sequence identity to the polypeptide of SEQ ID NO:
- 1 and comprising one or more mutations in the segment corresponding to residues 258 to 272 in the polypeptide of SEQ ID NO:
- 1, wherein the variant has an increased catalytic activity in converting N-acetylserotonin to melatonin as compared to the polypeptide of SEQ ID NO:
- 1 and comprises an amino acid substitution in one or more residues corresponding to residues 258, 260, and 272 in the polypeptide of SEQ ID NO:
- 1, wherein the amino acid substitutions correspond to A258E, G260D, or T272A in the polypeptide of SEQ ID NO:
- 1.
A recombinant host cell comprising a nucleic acid encoding a variant of Homo sapiens acetylserotonin O-methyltransferase (ASMT), the variant having at least 98% sequence identity to the polypeptide of SEQ ID NO:
- 1 and comprising one or more mutations in the segment corresponding to residues 258 to 272 in the polypeptide of SEQ ID NO:
- 1, wherein the variant has an increased catalytic activity in converting N-acetylserotonin to melatonin as compared to the polypeptide of SEQ ID NO:
- 1 and comprises a mutation in one or more residues corresponding to residues 258, 260, or 272 in the polypeptide of SEQ ID NO:
- 1.
A recombinant host cell comprising a nucleic acid encoding a variant of Homo sapiens ASMT, the variant having at least 92% sequence identity to the polypeptide of SEQ ID NO:
- 1 and comprising one or more mutations in the segment corresponding to residues 258 to 272 in the polypeptide of SEQ ID NO:
- 1, wherein the variant has an increased catalytic activity in converting N-acetylserotonin to melatonin as compared to the polypeptide of SEQ ID NO:
- 1 and comprises an amino acid substitution in one or more residues corresponding to residues 258, 260, and 272 in the polypeptide of SEQ ID NO:
- 1, wherein the amino acid substitutions correspond to A258E, G260D, or T272A in the polypeptide of SEQ ID NO:
- 1.
A recombinant host cell comprising a nucleic acid encoding a variant of Homo sapiens acetylserotonin O-methyltransferase (ASMT), the variant having at least 99% sequence identity to the polypeptide of SEQ ID NO:
- 1 and comprises an amino acid substitution corresponding to A258E in the polypeptide of SEQ ID NO:
- 1, said variant having at least 50% increased catalytic activity in converting N-acetylserotonin to melatonin as compared to the polypeptide of SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.