Methods and compositions for production of aromatic and other compounds in yeast
AssigneeIowa State University Research Foundation, Inc.
InventorsZengyi Shao, Mingfeng Cao, Miguel Suastegui, Meirong Gao
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Abstract
The present disclosure includes methods and components for production of valuable industrial compounds in yeast. In an embodiment, the present invention provides a nucleic acid construct with increased stability for gene expression or gene editing comprising: a nucleic acid sequence encoding one or more of SEQ ID NO: 1-8 (CENs 1-8); and one or more regulatory elements functional in a yeast cell. In an embodiment of the present invention the nucleic acid constructs are vectors, preferably episomal vectors. High expression promoters, as well as methods for increasing production of compounds such as aromatics are disclosed.
Claims12
A modified recombinant yeast cell for producing an aromatic compound, comprising
- a) an expression system that comprises at least one nucleotide sequence that encodes a transketolase, TKL1, capable of being expressed;
- b) an expression system that comprises at least one nucleotide sequence that encodes a pentafunctional protein for aromatic amino acid biosynthesis (ARO1), wherein the nucleotide sequence has the mutation aro1D920A, capable of being expressed;
- wherein the expression of a protein involved in regulating the efficient localization of Golgi network proteins (RIC1), a pentafunctional protein for aromatic amino acid biosynthesis (ARO1) and a 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase (ARO4) are downregulated or absent, and wherein said modified yeast cell produces higher titers of aromatic compounds compared to wild-type yeast cell of the same strain.
Dependencies are inferred from the claim text.