Back to results
US 11,332,740 B2Granted
Methods for seamless nucleic acid assembly
AssigneeTwist Bioscience Corporation
InventorsRebecca Nugent, Siyuan Chen, Elian Lee, Nathan Raynard
Life of this patent
Estimating the term…
Abstract
Provided herein are methods, systems, and compositions for seamless nucleic acid assembly. Methods, systems, and compositions as provided herein provide for efficient assembly of nucleic acids without primer removal. Methods, systems, and compositions for seamless nucleic acid assembly comprise use of an endonuclease or exonuclease, optionally in conjunction with additional enzymes to assemble nucleic acids or polynucleotides.
Claims17
A method for nucleic acid assembly comprising:
- (a) providing at least 10 different nucleic acid fragments, wherein each of the at least 10 different nucleic acid fragments does not comprise a terminal region of sequence homology to another fragment of the at least 10 different nucleic acid fragments;
- and
- (b) mixing the at least 10 different nucleic acid fragments with a plurality of enzymes, wherein the plurality of enzymes is selected from an endonuclease, exonuclease, a polymerase, and a ligase to form a nucleic acid;
- and
- wherein the endonuclease is a flap endonuclease.
2 more elements
Dependencies are inferred from the claim text.