US 11,332,739 B2 · Porcine reproductive and respiratory syndrome virus cDNA clone and uses thereof

Abstract

The present invention relates to a method for preparing cDNA infectious clones based on the genome of an attenuated Porcine Reproductive and Respiratory Syndrome virus (PRRSV) and uses thereof for preparing efficacious and safety-enhanced vaccines against PRRSV. The invention further comprises an infectious cDNA clone obtainable by such method. The invention further provides an attenuated modified PRRSV, and immunogenic compositions and vaccines comprising that attenuated PRRSV. The present invention further provides the attenuated PRRSV for use in the prophylaxis and/or the treatment of PRRSV infections, and the attenuated PRRSV for use as vaccine or medicament in the prophylaxis and/or the treatment of PRRSV infections.

Claims18

Dependent claims

A method for generating an infectious cDNA clone based on the genome of an attenuated PRRSV strain, the method comprising:

a) identifying polymorphic zones of the genome sequence of a population of quasispecies of an attenuated strain of PRRSV,
b) determining the most frequent sequence within the polymorphic zones of the quasispecies population identified in step a), and
c) constructing an infectious cDNA clone comprising the most frequent sequence in at least one of the polymorphic zones identified in step a).

Dependencies are inferred from the claim text.

Facts

Application

16/322,316Filed 31 Jul 2017

Priority

EP 163823915 Aug 2016

Published

US 2019/0177721 A113 Jun 2019

Granted

17 May 2022

Term ends

Not estimatedThe source records don’t support an estimate.

Classification15 codes

C12N 15/1096
Processes for the isolation, preparation or purification of DNA or RNA: cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
A61K 39/12
Medicinal preparations containing antigens or antibodies: viral antigens
A61P 31/14
Antivirals: for RNA viruses
C07K 14/005
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from viruses

Porcine reproductive and respiratory syndrome virus cDNA clone and uses thereof

Life of this patent

Porcine reproductive and respiratory syndrome virus cDNA clone and uses thereof

Life of this patent

Abstract

Claims18