Loading patent…
AssigneeEnzymaster (Ningbo) Bio-Engineering Co., Ltd
InventorsHaibin Chen, Yong Koy Bong, Wenhui Zheng, Zhaoqi Zhang, Kuifang He, Chengxiao Zhang, Lei Sun, Yingxin Zhang
Estimating the term…
The present invention provides amino acid sequences of engineered decarboxylase polypeptides that are useful for catalyzing the decarboxylation of L-aspartate to produce β-alanine, and the preparation process of engineered decarboxylase polypeptides as well as reaction process under industrial-relevant conditions. The present disclosure also provides polynucleotide sequences encoding engineered decarboxylase polypeptides, engineered host cells capable of expressing engineered decarboxylase polypeptides, and methods of producing β-alanine using the engineered cells. Compared to the wild-type decarboxylase, the engineered decarboxylase polypeptide provided by the invention has better activity and stability, and overcomes the inhibition by L-aspartic acid and/or β-alanine. The use of the engineered polypeptides of the present invention for the preparation of β-alanine results in higher unit activity, lower cost, and has good industrial application prospects.
An engineered decarboxylase polypeptide that catalyzes the decarboxylation of L-aspartic acid to produce β-alanine, wherein said polypeptide comprises an X109T substitution relative to SEQ ID NO:2, further wherein said amino acid sequence is selected from the group consisting of SEQ ID NOs:
An engineered decarboxylase polypeptide that catalyzes the decarboxylation of L-aspartic acid to produce β-alanine, wherein said polypeptide comprises the amino acid sequence set forth in SEQ ID NO:
Dependencies are inferred from the claim text.