Compositions comprising extracellular matrix of primitive animal species and related methods

A method of producing regenerative extracellular matrix (rECM) composition, comprising:

• obtaining tissue that has inherent regenerative capability, or that is undergoing regeneration, growth, or development, from an animal selected from the group consisting of:
• a lizard, an echinoderm, a planarian, a hydra, a bat, a spiny mouse, a land slug, a sea snail, an octopus, a cricket, a spider, a crab, a lobster, a zebrafish, or an anemone;
• freezing and thawing the tissue that has inherent regenerative capability or that is undergoing regeneration, growth, or development, to kill cells within the tissue;
• treating the tissue with one or more nucleases to digest nucleic acids in the tissue to produce rECM;
• and
• washing the nuclease-digested tissue in a solution, to remove cellular debris.