Conversion of biomass to useful intermediates
AssigneeAlliance for Sustainable Energy, LLC
InventorsGregg Tyler Beckham, Christopher W. Johnson, Derek R. Vardon
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Abstract
An aspect of the present disclosure is a microbial cell that includes a genetic modification resulting in the expression of a deficient form of an endogenous dioxygenase, and a gene encoding an exogenous dioxygenase and a promoter sequence, where the endogenous dioxygenase includes PcaH and PcaG, the exogenous dioxygenase includes LigA and LigB, the microbial cell is capable of growth utilizing at least one of a cellulose decomposition molecule or a lignin decomposition molecule, and the microbial cell is capable of producing 2-hydroxy-2H-pyran-4,6-dicarboxylic acid.
Claims9
A non-naturally occurring Pseudomonas capable of producing 2-oxo-2H-pyran-4,6-dicarboxylic acid, comprising the deletion of genes encoding a protocatechuate 3,4-dioxygenase and further comprising insertion of genes encoding a protocatechuate 4,5-dioxygenase and a 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase wherein the Pseudomonas is capable of growing on at least one of a cellulose decomposition molecule or a lignin decomposition molecule.
Dependencies are inferred from the claim text.