# US 11,326,180 B2 · Biological control of cucumber green mottle mosaic virus > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 11,326,180 B2 - Document ID: US11326180B2 - Status: Granted - Kind: B2 - Application number: 17/017,992 - Filed: 2020-09-11 - Published: 2020-12-31 - Granted: 2022-05-10 - Earliest claimed: 2018-09-14 - Assignees: A&L Canada Laboratories Inc. - Applicants: A&L Canada Laboratories, Inc. - Inventors: Keri Wang; George Lazarovits; Yibin Liu; Magda Konopka; Greg Patterson - Web page: https://patentgrep.com/patent/US11326180B2 ## CPC - A01H 6/346: {Cucumis sativus[cucumber]} - C12N 15/8283: {for virus resistance} - C12N 7/00: Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof (preparing medicinal viral antigen or antibody compositions, e.g. virus vaccines, A61K39/00) - C12N 2770/00021: Viruses as such, e.g. new isolates, mutants or their genomic sequences - C12N 2770/00062: by genetic engineering - C12N 2770/14021: Viruses as such, e.g. new isolates, mutants or their genomic sequences - A01H: NEW PLANTS OR {NON-TRANSGENIC} PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES - C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00) ## Abstract An attenuated strain of cucumber green mottle mosaic virus (CGMMV) is useful to protect cucumber plants from infection with the wild-type infectious CGMMV strain. The genome of the attenuated virus contains at least one mutation or group of mutations selected from c.4969G>A, c.3334C>T, and a group of at least six of the mutations c.315G>A; c.1498A>G; c.1660C>T; c.3430C>T; c.3528A>G; c.4144C>T; c.4248C>T; and c.6228C>T. These mutated genomes encode one or more mutations selected from R163711 in the 186 kDa readthrough replication protein, A1092V in the 129 kDa replication protein and/or the 186 kDa readthrough replication protein, and at least six mutations selected from G86S, E480G, S534F, A1124V, N1157D, P1362L, P1397S in the 129 kDa replication protein and/or the 186 kDa readthrough replication protein, and the A156V mutation in the coat protein. ## Claims ### Claim 1 A polydeoxyribonucleotide having a sequence functionally equivalent to a sequence of a genome of an attenuated strain of cucumber green mottle mosaic virus (CGMMV), wherein the sequence of the polydeoxyribonucleotide is a variant of SEQ ID NO:18 comprising at least one of the following options a), b) and c): a) A at the position corresponding to position 4969 of SEQ ID NO:18; b) T at the position corresponding to position 3334 of SEQ ID NO:18; and c) at least six nucleic acid bases selected from: A at the position corresponding to position 315 of SEQ ID NO:18; G at the position corresponding to position 1498 of SEQ ID NO:18; T at the position corresponding to position 1660 of SEQ ID NO:18; T at the position corresponding to position 3430 of SEQ ID NO:18; G at the position corresponding to position 3528 of SEQ ID NO:18; T at the position corresponding to position 4144 of SEQ ID NO:18; T at the position corresponding to position 4248 of SEQ ID NO:18; and T at the position corresponding to position 6228 of SEQ ID NO:18. ### Claim 2 (depends on 1) The polydeoxyribonucleotide according to claim 1, wherein the sequence of the polydeoxyribonucleotide comprises one or more of A at the position corresponding to position 4969 of SEQ ID NO:18 and T at the position corresponding to position 3334 of SEQ ID NO:18. ### Claim 3 (depends on 1) The polydeoxyribonucleotide according to claim 1, wherein the sequence of the polydeoxyribonucleotide comprises: A at the position corresponding to position 315 of SEQ ID NO:18; G at the position corresponding to position 1498 of SEQ ID NO:18; T at the position corresponding to position 1660 of SEQ ID NO:18; T at the position corresponding to position 3430 of SEQ ID NO:18; G at the position corresponding to position 3528 of SEQ ID NO:18; T at the position corresponding to position 4144 of SEQ ID NO:18; T at the position corresponding to position 4248 of SEQ ID NO:18; and T at the position corresponding to position 6228 of SEQ ID NO:18. ### Claim 4 (depends on 3) The polydeoxyribonucleotide according to claim 3, wherein the sequence of the polydeoxyribonucleotide comprises one or more of A at the position corresponding to position 4969 of SEQ ID NO:18 and Tat the position corresponding to position 3334 of SEQ ID NO:18. ### Claim 5 (depends on 1) The polydeoxyribonucleotide according to claim 1, wherein the sequence of the polydeoxyribonucleotide is selected from SEQ ID NO:42, SEQ ID NO:45, SEQ ID NO:48, SEQ ID NO:53, SEQ ID NO:58, SEQ ID NO:60, and variants thereof. ### Claim 6 (depends on 1) The polydeoxyribonucleotide according to claim 1, wherein the sequence of the polydeoxyribonucleotide is selected from SEQ ID NO:42, SEQ ID NO:45, SEQ ID NO:48, SEQ ID NO:60, and variants thereof. ### Claim 7 (depends on 1) The polydeoxyribonucleotide according to claim 1, wherein the polydeoxyribonucleotide encodes one or more proteins selected from: a 129 kDa protein having an amino acid sequence selected from SEQ ID NO:43, SEQ ID NO:46, SEQ ID NO:49, SEQ ID NO:54, SEQ ID NO:61 and variants thereof; a 186 kDa protein having an amino acid sequence selected from SEQ ID NO:44, SEQ ID NO:47, SEQ ID NO:50, SEQ ID NO:55, SEQ ID NO:59, SEQ ID NO:62 and variants thereof; and a coat protein having an amino acid sequence selected from SEQ ID NO:32 and variants thereof. ### Claim 8 (depends on 1) A vector comprising the polydeoxyribonucleotide according to claim 1. ### Claim 9 (depends on 1) A genetically modified cell, wherein the cell expresses the polydeoxyribonucleotide according to claim 1. ### Claim 10 (depends on 9) The genetically modified cell according to claim 9, wherein the cell is a microorganism. ### Claim 11 (depends on 9) The genetically modified cell according to claim 9, wherein the cell is a plant cell. ### Claim 12 (depends on 9) A composition for preventing symptoms associated with infection by wild-type CGMMV in a plant or for increasing resistance of a plant to infection by wild-type CGMMV, wherein the composition comprises a genetically modified cell according to claim 9 and an agriculturally acceptable carrier. ### Claim 13 (depends on 9) A method for preventing symptoms associated with infection by wild-type CGMMV in a plant or for increasing resistance of a plant to infection by wild-type CGMMV, wherein the method comprises inoculating the plant with a genetically modified cell according to claim 9. ### Claim 14 (depends on 12) A method for preventing symptoms associated with infection by wild-type CGMMV in a plant or for increasing resistance of a plant to infection by wild-type CGMMV, wherein the method comprises inoculating the plant with a composition according to claim 12.